ART OF MEDICINE 2017

 

THE ROLE OF THE CELLULAR IMMUNE DEFENSE OF THE ORGANISM AT THE EARLY STAGE OF THE EXPERIMENTAL BACTERIAL-IMMUNE PERIODONTITIS DEVELOPMENT

 

A.Ye. Demkovych

          I. Horbachevsky Ternopil State Medical University, Department of Prosthetic Dentistry, Ternopil, Ukraine e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

  

Abstract

Abstract. The article presents the results of research on the parameters of cellular immune protection determined by the relative number of CD3+, CD4+, CD8+, CD19+, CD16+ and immunoregulatory index (CD4+/CD8+) markers in intact animals, and on the 7th day of the experimental bacterial-immune periodontitis. The study was conducted under vivarium conditions on white, non-breeding, clinically healthy male rats. Experimental animals were divided into two groups: the first group – intact animals (n = 10); the second group is animals with experimental periodontitis on the 7th day of the study (n = 8). Experimental bacterial-immunode periodontitis in experimental animals was induced by introducing into the tissue of the periodontal complex a mixture of microorganisms diluted with egg protein. Indicators of cellular immunity were determined by the method based on the interaction of monoclonal antibodies labeled with a fluorescence label with surface antigens of the lymphocytes. The obtained results were statistically analyzed using parametric and nonparametric statistical methods. Attention is drawn to the characteristic change in the indicators of cell immunity at the early stage of the development of the experimental periodontitis. In particular, in the period of acute manifestations of inflammatory response, at 7th day, there was an increase in the relative number of CD8+, CD16+ cells in the blood, and the content of CD3+, CD4+, CD19+ changed in the opposite direction, ie, decreased. In this case, the immunoregulatory index (CD4+/CD8+) as an important indicator of immunological activity was reduced. Having investigated the functional state of cellular immune defense in white rats with simulated bacterial-immune periodontitis, there was a violation of the functioning of the T-cell line of the immune system, as evidenced by a decrease in the percentage of B- and T-cells due to T-helper cells, with an increase in the T-killers in the early period of development of inflammatory reaction in the periodontal complex.

 

Key words. bacterial-immune periodontitis, immune system, T-lymphocytes, B-lymphocytes, inflammation.

 

Full text: PDF (Rus)

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