ART OF MEDICINE 2017

 

CONTENT OF MINERAL ELEMENTS IN HYPATOCYTES OF RATS WHO HAVE INSULIN RESISTANCE, OBESITY AND OBESITY IN COMBINATION WITH IODIDEFICIATE

 

I.Hlozhyk

          Docent of Department of Biochemistry and Hygiene Lviv State University of Physical Culture e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

  

Abstract

Abstract. The article covers the topic of mineral elements in hepatocytes of rats with insulin resistance, obesity and obesity in combination with iodine deficiency.

According to modern concepts, Ir and obesity are the root pathophysiological processes that lead to the cascade of pathological reactions. The result of that reactions is the formation of a whole set of disorders and diseases. That’s why it is crucial to study fundamental mechanisms of the development of that disorders. Studying of the pathogenesis of Ir and obesity in the terms of the state of macro- and micronutrient provision, understanding of the peculiarities of pathogenetic and therapeutic significance of the level of macro- and microelements - can be the basis for the development of new methods of prevention and treatment of these pathologies.

Animals with Ir have a 27.3% decrease of the copper part in the liver. In the group of animals with O and O + Io, we observe an 27.4% (p<0,05) and 36.2% increase of the current element in comparison to the absolutely healthy animals. The group of animals with IR has the 34.2% higher level of iron in comparison to the standart, animals with O have the same level as in the previous group (34.8%), in the group of animals with O + Io the percentage is 38, 4% (p<0,05).  As for the calcium part in the liver homogenates of animals with Ir, it is 28.4% (p<0,05) higher in comparison to the standart, in animals with O up to 22.2% (p<0,05), and by O + Io - 23.1% (p<0,05).The content of magnesium in animals with Ir is 19.3% (p<0,05) lower in comparison to the standart, in animals with O the percentage is 27.2% and in the group of animals О + Io  - 28.1% (p<0,05). As for Zinc, there was no significant fluctuations in the content of this element. Animals with acquired Ir and O, O + Io, have 10%, 18.4% and 23.5%  respectively lower percentage in comparison to the normal rate. The content of Mangan in animals with Ir was 13.6% lower than the standart in the group with О - 14.8%, and the content of this microelement decreased by 16.2%. Regarding Chromium, we found a probable decrease in the concentration of this microelement in animals of the Ir group by 52.3%, in animals from the group of O - 58.1% and in the group of animals with O + Io - 56.2% (p<0,05).

Reducing the copper content in the group of animals with the Ir can be explained by the fact that as a result of the development of pathological process in the liver (inflammation, destruction of hepatocytes) in the blood there is an outlet of substances containing copper. It is known that in chronic liver disease, the content of copper in the serum increases. Reducing the copper content may lead to a decrease in the activity of copper-dependent metal enzymes, and, as a result, to enhance the processes of lipid peroxidation (oxidation of lipids), oxidative modification of proteins, nucleic acid degradation, cytokine release, functional state disorders, and development of pathological process. The increase in the content of this trace element in the groups of animals with O and O + Io is probably due to the expression of Zn, Cu-containing superoxide dismutase, and metal-ionone. It is known from literary sources that the pathological process in the liver may lead to changes in the metabolism of iron. Considering that the main function of iron is its participation in oxidative-reduction reactions, the result can be explained by the increase of oxidative processes in hepatocytes. It can be assumed that the increase of the level of calcium in hepatocytes leads to the launch of the mitochondrial apoptotic signaling cascade, since this cation plays an important role in the mechanisms of death of liver cells. As it is known from literary sources, the development of insulin resistance is associated, above all, with the deficiency of magnesium. According to clinical and fundamental studies, Magnesium has a hepatoprotective effect. Removal of magnesium from hepatocytes may be associated with a decrease in the content of ATP. It was investigated that magnesium ions act as the essential cofactors of many enzymes involved in the metabolism of carbohydrates and fats, while its low level increases the index of atherogenicity, which is a factor of alimentary obesity. Probably, the violation of hepatocyte metabolism leads to the generation of AFK, which causes the release of zinc from proteins, resulting in their dysfunction. The results can be explained by the increased use of zinc in the production of zincall2-glycoprotein, which increases with alimentary obesity, having, according to scientists, a protective role. Mangan activates the enzymes of the citric acid cycle, enhances the action of insulin necessary for its synthesis. Since Mangan is an important bioelement that strengthens the hypoglycemic effect of insulin, counteracts fatty liver degeneration, the tendency to determine its content in animals of all experimental groups can be explained by the development of pathological process in hepatocytes. Violation of metabolism in the liver causes a rapid decrease in the content of Chromium.

The achieved results demonstrate changes in the content of mineral elements in animal groups with insulin resistance, obesity and obesity in combination with iodine deficiency. Further research of the features of metabolism of bioelements in the liver tissue will lead to the better understanding of the biochemical mechanisms of the development of these pathologies. Furthermore, it will create opportunities for a comprehensive correction of other metabolic changes.

 

Key words: mineralelements, insulinresistance, obesity, iodinedeficiency.

 

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